Differential effects of long-chain (sphingoid) bases on the monocytic differentiation of human leukemia (HL-60) cells induced by phorbol esters, 1 alpha, 25-dihydroxyvitamin D3, or ganglioside GM3

• Published in: Cancer research (Chicago, Ill.) Vol. 49; no. 12; p. 3229
• Main Authors: Stevens, V L, Winton, E F, Smith, E E, Owens, N E, Kinkade, Jr, J M, Merrill, Jr, A H
• Format: Journal Article
• Language: English
• Published: United States 15.06.1989
• Subjects: Antigens, Neoplasm - analysis; Antigens, Surface - analysis; Calcitriol - pharmacology; Cell Differentiation - drug effects; Cell Division - drug effects; Cell Line; Cell Survival - drug effects; G(M3) Ganglioside - pharmacology; Gangliosides - pharmacology; Humans; Kinetics; Leukemia, Promyelocytic, Acute; Sphingosine - analogs & derivatives; Sphingosine - pharmacology; Tetradecanoylphorbol Acetate - pharmacology; Tumor Cells, Cultured - cytology; Tumor Cells, Cultured - drug effects

Conditions were developed to prolong the ability of sphinganine, a potent inhibitor of protein kinase C, to block the phorbol ester-induced adherence of HL-60 cells beyond 24 h. The loss of inhibition after this time seen previously (A.H. Merrill, Jr., A.M. Sereni, V.L. Stevens, Y.A. Hannun, R.M. Be...