Acid proteinase of chicken liver. Purification and properties (author's transl)
A method for purifying the haemoglobinolitic activity to acid pH in chicken liver is described. The purified preparation contains cathespin D activity since it is inhibited by diazoacetyl-DL-norleucine methylester in the presence of cupric ions, while thiol-enzyme reagents do not affect it. The mole...
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Published in | Revista espanola de fisiologia Vol. 36; no. 1; p. 63 |
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Main Authors | , , |
Format | Journal Article |
Language | Spanish |
Published |
Spain
01.03.1980
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Subjects | |
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Abstract | A method for purifying the haemoglobinolitic activity to acid pH in chicken liver is described. The purified preparation contains cathespin D activity since it is inhibited by diazoacetyl-DL-norleucine methylester in the presence of cupric ions, while thiol-enzyme reagents do not affect it. The molecular weight of the enzyme is approximately 40,000--45,000 and the pH optimum against haemoglobin and bovine serum albumin is 3.5-3.7. The rate of degradation of albumin and casein is much less than that of haemoglobin. Cathepsin D preparations with different purification degrees, exhibit a similar percentage of activation at acid pH; the highest activation is observed at pH 3.1-3.3. |
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AbstractList | A method for purifying the haemoglobinolitic activity to acid pH in chicken liver is described. The purified preparation contains cathespin D activity since it is inhibited by diazoacetyl-DL-norleucine methylester in the presence of cupric ions, while thiol-enzyme reagents do not affect it. The molecular weight of the enzyme is approximately 40,000--45,000 and the pH optimum against haemoglobin and bovine serum albumin is 3.5-3.7. The rate of degradation of albumin and casein is much less than that of haemoglobin. Cathepsin D preparations with different purification degrees, exhibit a similar percentage of activation at acid pH; the highest activation is observed at pH 3.1-3.3. |
Author | Barceló, F Vives, N Bozal, J |
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Snippet | A method for purifying the haemoglobinolitic activity to acid pH in chicken liver is described. The purified preparation contains cathespin D activity since it... |
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SubjectTerms | Animals Chickens - metabolism Hydrogen-Ion Concentration Liver - enzymology Peptide Hydrolases - isolation & purification |
Title | Acid proteinase of chicken liver. Purification and properties (author's transl) |
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