An approach to the in vitro study of the UTP/UDPglucose/UDP moiety-conserved cycle

• Published in: Biochimica et biophysica acta Vol. 1200; no. 3; p. 297
• Main Authors: Fassy, F, Hervagault, J F
• Format: Journal Article
• Language: English
• Published: Netherlands 18.08.1994
• Subjects: Animals; Glycogen Synthase - metabolism; Kinetics; Models, Chemical; Nucleoside-Diphosphate Kinase - metabolism; Rabbits; Uridine Diphosphate - metabolism; Uridine Diphosphate Glucose - metabolism; Uridine Triphosphate - metabolism; UTP-Glucose-1-Phosphate Uridylyltransferase - metabolism

The kinetic behavior of a moiety-conserved ternary cycle is tested experimentally. This system contains the enzymes UDPglucose pyrophosphorylase, glycogen synthase and nucleoside diphosphokinase, converting respectively UTP into UDPglucose, then into UDP and back to UTP in a cyclic manner. The UDPGl...