Interaction of catalytically active antibodies with oligoribonucleotides

The interaction of antibodies from blood sera of patients with autoimmune pathology, systemic lupus erythematosus with oligoribonucleotides was studied. The RNA-hydrolyzing activity was shown to be an intrinsic property of autoantibodies. Enzymic activity of antibodies in hydrolysis of poly(U) was e...

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Bibliographic Details
Published inMolekuliarnaia biologiia Vol. 28; no. 4; p. 738
Main Authors Buneva, V N, Andrievskaia, O A, Romannikova, I V, Gololobov, G V, Iadav, R P, Iamkovoĭ, V I, Nevinskiĭ, G A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.1994
Subjects
Antibodies, Catalytic - immunology
Autoantibodies - immunology
Humans
Hydrogen-Ion Concentration
Hydrolysis
Lupus Erythematosus, Systemic - immunology
Oligoribonucleotides - immunology
Oligoribonucleotides - metabolism
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Summary:The interaction of antibodies from blood sera of patients with autoimmune pathology, systemic lupus erythematosus with oligoribonucleotides was studied. The RNA-hydrolyzing activity was shown to be an intrinsic property of autoantibodies. Enzymic activity of antibodies in hydrolysis of poly(U) was estimated at 20-40% of that of RNase A. In contrast to known eukaryotic RNases, the autoantibodies possess a specific RNA-hydrolyzing activity for oligo r(A). The RNA-nicking activity of antibodies in hydrolysis of oligoadenylates was more higher than with hydrolysis of oligo d(A). Optimal conditions of r(pA)13 hydrolysis were selected, including the optimal of pH = 8.7.
ISSN:0026-8984