Effect of dicarboxylic (C6 and C9) acids on a human squamous carcinoma cell line in culture
In tissue culture, azelaic acid (C9) has been shown to have an anti-proliferative and cytotoxic effect on human and murine malignant melanocytes, with inhibition of mitochondrial oxido-reductase enzymes and DNA synthesis, and damage to mitochondria. Recent reports of effects on differentiation of no...
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Published in | Histology and histopathology Vol. 4; no. 2; p. 167 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Spain
01.04.1989
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Abstract | In tissue culture, azelaic acid (C9) has been shown to have an anti-proliferative and cytotoxic effect on human and murine malignant melanocytes, with inhibition of mitochondrial oxido-reductase enzymes and DNA synthesis, and damage to mitochondria. Recent reports of effects on differentiation of normal keratocytes have led to the present study of its effects on a squamous carcinoma cell line. Cells were exposed to single doses of disodium salts of azelaic (C9(2)Na) and adipic (C6(2)Na) acids at concentrations of 10(-2)M and 5 x 10(-2)M for 48 hrs. Only C9(2)Na at 5 x 10(-2) M for 4 hrs., and longer, significantly affected proliferation, and the cells exhibited massive swelling of mitochondria with loss of cristae. The results further confirm the probable value of azelaic acid as a general anti-tumoral agent rather than a specifically melanocytotoxic one. They could justify clinical studies on the effect of topical azelaic acid therapy on squamous cell carcinoma in vivo. |
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AbstractList | In tissue culture, azelaic acid (C9) has been shown to have an anti-proliferative and cytotoxic effect on human and murine malignant melanocytes, with inhibition of mitochondrial oxido-reductase enzymes and DNA synthesis, and damage to mitochondria. Recent reports of effects on differentiation of normal keratocytes have led to the present study of its effects on a squamous carcinoma cell line. Cells were exposed to single doses of disodium salts of azelaic (C9(2)Na) and adipic (C6(2)Na) acids at concentrations of 10(-2)M and 5 x 10(-2)M for 48 hrs. Only C9(2)Na at 5 x 10(-2) M for 4 hrs., and longer, significantly affected proliferation, and the cells exhibited massive swelling of mitochondria with loss of cristae. The results further confirm the probable value of azelaic acid as a general anti-tumoral agent rather than a specifically melanocytotoxic one. They could justify clinical studies on the effect of topical azelaic acid therapy on squamous cell carcinoma in vivo. |
Author | Pätzold, H C Daridan, M E Picardo, M Ethridge, L B Bhasin, Y P Nazzaro-Porro, M Breathnach, A S Passi, S Robins, E J |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/2520453$$D View this record in MEDLINE/PubMed |
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Snippet | In tissue culture, azelaic acid (C9) has been shown to have an anti-proliferative and cytotoxic effect on human and murine malignant melanocytes, with... |
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SubjectTerms | Adipates - pharmacology Antineoplastic Agents - pharmacology Carcinoma, Squamous Cell - ultrastructure Cell Division - drug effects Cell Survival - drug effects Dicarboxylic Acids - pharmacology Humans Microscopy, Electron Mitochondrial Swelling - drug effects Tumor Cells, Cultured |
Title | Effect of dicarboxylic (C6 and C9) acids on a human squamous carcinoma cell line in culture |
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