Genetic dissection of a Leishmania flagellar proteome demonstrates requirement for directional motility in sand fly infections

• Published in: bioRxiv
• Main Authors: Beneke, Tom, Demay, Francois, Hookway, Edward, Ashman, Nicole, Jeffery, Heather, Smith, James, Valli, Jessica, Becvar, Tomas, Myskova, Jitka, Lestinova, Tereza, Shahaan Shafiq, Sadlova, Jovana, Volf, Petr, Wheeler, Richard J, Gluenz, Eva
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 27.11.2018
• Subjects: Amastigotes; CRISPR; Defecation; Flagella; Gastrointestinal tract; Leishmania; Life cycles; Mass spectroscopy; Motility; Phagocytes; Promastigotes; Protein composition; Proteins; Proteomes; Protozoa; Reproductive fitness; Swimming

The protozoan parasite Leishmania possesses a single flagellum, which is remodelled during the parasite's life cycle from a long motile flagellum in promastigote forms in the sand fly to a short immotile flagellum in amastigotes residing in mammalian phagocytes. This study examined the protein...