Optimization of critical parameters for immobilization of Streptomyces clavuligerus on alginate gel matrix for cephamycin C production

• Published in: World journal of microbiology & biotechnology Vol. 15; no. 2; pp. 167 - 173
• Main Authors: DEVI, S, SRIDHAR, P
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.04.1999
• Subjects: Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Immobilization of organelles and whole cells; Immobilization techniques; Methods. Procedures. Technologies; Streptomyces clavuligerus; Actinomycetales; Antibiotic; Streptomycetaceae; Support; Production; Immobilization; Bacteria; Actinomycetes; Streptomyces clavuligerus; Alginates; Entrapped microorganism; Optimization
• ISSN: 0959-3993

The optimum critical parameters for immobilization of Streptomyces clavuligerus on alginate gel matrix for cephamycin C production, i.e. sodium alginate (wt. %), CaCl sub(2) (M) and cell concentration (wt. %), curing time (h), for enhanced gel stability, were obtained employing a full factorial search. The results indicate that the concentrations of CaCl sub(2) and inoculum size were found to have a pronounced effect on cephamycin C fermentation. On the other hand, the higher concentration of sodium alginate exerted an adverse influence either individually or in combination with other variables. The path steepest ascent method has been used to optimize the variables. The optimum concentrations of matrix components were 3.218% sodium alginate, 0.996 M CaCl sub(2), 19.06% cell concentration and 17.16 h. of curing time supported higher cephamycin C production, at 48 h. of fermentation.