高温对水稻根际细菌群落及功能代谢多样性的影响

• Published in: Guanʻgai paishui xuebao Vol. 39; no. 11; pp. 97 - 103
• Main Authors: QIU Husen, ZHEN, Bo, ZHOU Xinguo
• Format: Journal Article
• Language: Chinese; English
• Published: Xinxiang City Chinese Academy of Agricultural Sciences (CAAS) Farmland Irrigation Research Institute Editorial Office of Journal of Irrigation and Drainage 01.11.2020; 中国农业科学院 农田灌溉研究所,河南 新乡453002; 中国农业科学院商丘农田生态系统国家野外科学观测研究站,河南商丘 476000
• Subjects: Amino acids; Annotations; Bacteria; Biogeochemical cycles; Biosynthesis; Carbon cycle; Catabolism; Cell death; Cell growth; Classification; Ecosystem services; Environmental stress; Functionals; Genes; Intracellular signalling; Latescibacteria; Lipid metabolism; Lipids; Metabolism; Metabolites; Microbial activity; Microorganisms; Next-generation sequencing; Nutrients; Phylogeny; Physiology; Protein biosynthesis; Protein structure; Protein synthesis; Protein turnover; Relative abundance; Rhizosphere; Rhizosphere microorganisms; Rice; Secondary metabolites; Signal transduction; Soil improvement; Soil microorganisms; Soil resistance; Soil stresses; Structure-function relationships; Temperature effects; Thermal resistance; Thermal stress; 功能基因; 高温胁迫; 水稻; 生理代谢; 根际细菌
• ISSN: 1672-3317
• DOI: 10.13522/j.cnki.ggps.2019160

【目的】基于高通量技术和功能预测，探究水稻根际细菌群落主要生理代谢过程基因表达水平及其对高温胁迫的响应特征。【方法】通过盆栽试验对拔节期水稻进行7 d的高温胁迫处理，提取根际土壤微生物DNA进行高通量测序，结合PICRUSt功能预测进行细菌群落分析、基因注释及功能分类。【结果】与对照相比，高温胁迫促进了一些丰度相对较低的细菌门类（如：芽孢单菌门、浮霉菌门、Latescibacteria、螺旋菌门、Microgenomates和Candidatus_Berkelbacteria）的富集，提高了细菌多样性和细菌菌群之间共生关系的比例。细菌中活性基因主要源于变形菌门，其次是酸杆菌门、放线菌门和绿弯菌门。基于COG功能分类发现，对照与高温胁迫处理土壤中表达量最高的基因均为新陈代谢相关的基因，其次是参与细胞加工和信号传递的基因。但高温胁迫提高了参与细胞运动、信号传导机制、胞内运转、分泌和囊泡运输和细胞壁/膜/器形成基因的相对丰度；却降低了参与氨基酸运输和代谢、脂质转运和代谢、次生代谢生物合成以及翻译的相关基因的丰度。【结论】高温胁迫虽未改变根际细菌菌群结构，但提高了细菌群落多样性，加强了细菌菌群的共生关系，显著影响参与细胞的周转，代谢以及蛋白质合成的基因表达。