Gene expression of the key enzymes controlling starch synthesis and metabolism in rice grain endosperm under effects of high temperature after anthesis

Taking an early-season indica cultivar 'Jiazao 935' whose grain quality was sensitive to temperature as test material, and by using artificial climatic chamber and real-time fluorescence quantitative PCR (FQ-PCR), this paper studied the relative expression amount and its dynamic changes of...

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Published inYing yong sheng tai xue bao Vol. 23; no. 3; p. 745
Main Authors Zhong, Lian-Jin, Dong, Hu, Cai, Xiao-Bo, Feng, Yan-Ning, Ren, Ping, Cheng, Fang-Min
Format Journal Article
LanguageChinese
Published China 01.03.2012
Subjects
1,4-alpha-Glucan Branching Enzyme - genetics
Edible Grain - metabolism
Endosperm - metabolism
Gene Expression Regulation, Plant
Hot Temperature
Oryza - enzymology
Oryza - genetics
Protein Isoforms - genetics
Starch - biosynthesis
Starch - metabolism
Stress, Physiological - physiology
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Summary:Taking an early-season indica cultivar 'Jiazao 935' whose grain quality was sensitive to temperature as test material, and by using artificial climatic chamber and real-time fluorescence quantitative PCR (FQ-PCR), this paper studied the relative expression amount and its dynamic changes of ten isoform genes of the key enzymes controlling starch synthesis and metabolism in rice grain endosperm, including sbe1, sbe3, and sbe4 of starch branching enzyme (SBE), isal, isa2, isa3, and pul of starch debranching enzyme (DBE), and Wx, sss1, and sss2a of starch synthase (SS), at the mean daily temperature 22 and 32 degrees C after anthesis. There existed obvious differences in the expression patterns of these genes under the high temperature stress, and the expression patterns were isoform-dependent. The relative expression amount of sbe1 and sbe3 under high temperature decreased significantly, and both of the genes were the sensitive isoform genes of SBE to high temperature stress. Among the DBE genes, pul was the iso
ISSN:1001-9332