Alpha-momordicin Regulates Hepatocyte Cytokine Expression

To investigate the regulation effect of α-momordicin (α-MMC) on the synthesis and secretion of cytokines in hepatocytes cells. Hepatocytes L02 were treated with 189 μg/mL α-MMC with culture supernatant and lysate samples were harvested in different timepoint. Expressions of T-helper 17 (TH17) cytoki...

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Published inSichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 50; no. 2; p. 193
Main Authors Hu, Da-Min, Shen, Fu-Bing, Tan, Xue-Ling, Zheng, Jue-Cun, Deng, Nian-Hua
Format Journal Article
LanguageChinese
Published China 01.03.2019
Subjects
CD40 Ligand
Cells, Cultured
Cytokines - metabolism
Hepatocytes - drug effects
Hepatocytes - metabolism
Humans
Sterols - pharmacology
Tumor Necrosis Factor-alpha
Liver cell L02
Regulation
Cytokine
α-momordicin
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Summary:To investigate the regulation effect of α-momordicin (α-MMC) on the synthesis and secretion of cytokines in hepatocytes cells. Hepatocytes L02 were treated with 189 μg/mL α-MMC with culture supernatant and lysate samples were harvested in different timepoint. Expressions of T-helper 17 (TH17) cytokine profile in samples were detected by the Bio-Plex 200 suspension chip assay system. Compared with 0 h, after the α-MMC treatment of L02 hepatocytes for 2 h, 4 h and 8 h, the intracellular synthesis of cytokines interleukin (IL)-1b, IL-6, IL-17A, IL-31, IL-33, soluble CD40 ligand (sCD40L), tumor necrosis factor-α (TNF-α) were all significantly decreased ( <0.05), and IL-6, IL-4, IL-17A, and sCD40L secreted into the extracellular fluid also decreased significantly ( <0.05). α-MMC can significantly inhibit the synthesis and secretion of cytokines such as IL-6, IL-17A and TNF-α in hepatocytes, which may become a side effect of its anti-tumor application.
ISSN:1672-173X