Effects of FBI-1 silencing on proliferation and apoptosis of triple-negative breast cancer cell line MDA-MB-231

• Published in: Sheng li hsüeh pao Vol. 70; no. 5; p. 497
• Main Authors: Chen, Mao-Jian, Wang, Li, Yang, Wei-Ping, Qin, Qing-Hong, Tan, Qi-Xing, Lian, Bin, Wei, Chang-Yuan
• Format: Journal Article
• Language: Chinese
• Published: China 25.10.2018
• Subjects: Animals; Apoptosis; bcl-2-Associated X Protein - metabolism; Caspase 3 - metabolism; Cell Line, Tumor; Cell Proliferation; DNA-Binding Proteins - genetics; Humans; Mice; Mice, Nude; Proto-Oncogene Proteins c-bcl-2 - metabolism; RNA Interference; RNA, Messenger; RNA, Small Interfering; Survivin - metabolism; Transcription Factors - genetics; Triple Negative Breast Neoplasms - genetics; Triple Negative Breast Neoplasms - pathology
• ISSN: 0371-0874

This work aimed to observe the effects of short hairpin RNA (shRNA)-silenced FBI-1 (factor that binds to the inducer of short transcripts of human immunodeficiency virus-1) on proliferation and apoptosis of triple-negative breast cancer cell line MDA-MB-231. qRT-PCR and Western blot analysis were applied to detect the mRNA and/or protein expression of FBI-1, Bcl-2, Bax, cleaved-Caspase 3 and Survivin. RNA interference method was used to silence FBI-1 expression in MDA-MB-231 cells. CCK-8 and colony formation assay were employed to detect the cell proliferation. Flow cytometry was employed for examining cell apoptosis. In vivo tumorigenicity of MDA-MB-231 cells was detected by tumor transplantation in nude mice. The results showed that the mRNA and protein expressions of FBI-1 were higher in MDA-MB-231 cells compared with those in normal human mammary epithelial cells MCF-10A. FBI-1 gene silencing inhibited proliferation and induced apoptosis of MDA-MB-231 cells in vitro, together with decreased Bcl-2 and Surv