AdipoRon for the treatment of type 2 diabetes in mice and its possible mechanism of the liver

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 32; no. 3; p. 198
• Main Authors: Xiao, Min, Qu, Xiao-Hu, Li, Chang-Xi, Lv, Ju-Ping, Shi, Yang, Xie, Ke-Jian
• Format: Journal Article
• Language: Chinese
• Published: China 08.03.2016
• Subjects: Alanine Transaminase; Alkaline Phosphatase; Animals; Aspartate Aminotransferases; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Type 2 - drug therapy; Liver - drug effects; Male; Mice; Mice, Inbred C57BL; Piperidines - pharmacology; liver cell injury; adiponectin receptor excitomotor; type 2 diabetes; mice
• ISSN: 1000-6834
• DOI: 10.13459/j.cnki.cjap.2016.03.002

To observe the effect of AdipoRon for the treatment of type 2 diabetes (T2DM)in mice and its effect on the liver. Forty male C57/BL6 mice (SPF) were randomly divided to normal control (NC) group and the experimental group. To establish the T2DM mice model, mice in the experimental group were fed with high fat and high glucose, combined with intraperitoneal injection of streptozotocin (STZ) in small doses, and mice were further subdivided into model control (DM) group, model control with low AdipoRon (DM+L) group and model control with high AdipoRon (DM+H) group ( =10). Serum indexes, such as levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) were detected biochemically and the morphological changes of liver cells were observed with HE staining and expression of liver carbohydrate related gene (PEPCK) were determined by real-time fluorescence quantitative PCR (real time FQ-PCR). Compared with mice in the DM group, levels of ALT, AST, ALP, triglyceride (TG), g