Establishment of a pulmonary metastasis model of melanoma with stably expressed hTERT/Luc
To construct the eukaryotic expression vectors of human telomerase reverse transcriptase (hTERT) and luciferase (Luc) gene, and stably transfect B16 cell line with them, and inoculate mice via caudal vein to construct pulmonary metastasis models. The hTERT and Luc cDNA fragments were inserted into e...
Saved in:
Published in | Xi bao yu fen zi mian yi xue za zhi Vol. 29; no. 1; p. 89 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | Chinese |
Published |
China
01.01.2013
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | To construct the eukaryotic expression vectors of human telomerase reverse transcriptase (hTERT) and luciferase (Luc) gene, and stably transfect B16 cell line with them, and inoculate mice via caudal vein to construct pulmonary metastasis models.
The hTERT and Luc cDNA fragments were inserted into eukaryotic expression vectors to obtain the recombinant plasmids pIRES-neo-hTERT and pIRES-hyg3-Luc. The plasmids were then transfected into B16 cells by Lipofectamine(TM); 2000. Afte the stably transfected cell line was screened by G418 and hygromycin B, the transcription and expression of the hTERT and Luc genes were identified by Western blotting and immunofluorescence staining. The stably transfected cells were inoculated via caudal vein into male C56BL/6 mice to construct pulmonary metastasis models. An in vivo imaging system was used to monitor the growth and metastasis of the tumors.
A stably transfected cell line was established and the express rates of hTERT and Luc gene in the stable cell line were 84% and |
---|---|
AbstractList | To construct the eukaryotic expression vectors of human telomerase reverse transcriptase (hTERT) and luciferase (Luc) gene, and stably transfect B16 cell line with them, and inoculate mice via caudal vein to construct pulmonary metastasis models.
The hTERT and Luc cDNA fragments were inserted into eukaryotic expression vectors to obtain the recombinant plasmids pIRES-neo-hTERT and pIRES-hyg3-Luc. The plasmids were then transfected into B16 cells by Lipofectamine(TM); 2000. Afte the stably transfected cell line was screened by G418 and hygromycin B, the transcription and expression of the hTERT and Luc genes were identified by Western blotting and immunofluorescence staining. The stably transfected cells were inoculated via caudal vein into male C56BL/6 mice to construct pulmonary metastasis models. An in vivo imaging system was used to monitor the growth and metastasis of the tumors.
A stably transfected cell line was established and the express rates of hTERT and Luc gene in the stable cell line were 84% and |
Author | Zhang, Liang Wang, Xiaoxiong Gao, Jiangping Yan, Jinqi Wang, Yu Yu, Jiyun Xu, Yuanji Gao, Kun Xiao, Yi |
Author_xml | – sequence: 1 givenname: Yi surname: Xiao fullname: Xiao, Yi email: xiaoyi1007@gmail.com organization: Department of Urology, Chinese PLA General Hospital, Beijing 100853, China. xiaoyi1007@gmail.com – sequence: 2 givenname: Jiangping surname: Gao fullname: Gao, Jiangping – sequence: 3 givenname: Kun surname: Gao fullname: Gao, Kun – sequence: 4 givenname: Jinqi surname: Yan fullname: Yan, Jinqi – sequence: 5 givenname: Liang surname: Zhang fullname: Zhang, Liang – sequence: 6 givenname: Yu surname: Wang fullname: Wang, Yu – sequence: 7 givenname: Yuanji surname: Xu fullname: Xu, Yuanji – sequence: 8 givenname: Xiaoxiong surname: Wang fullname: Wang, Xiaoxiong – sequence: 9 givenname: Jiyun surname: Yu fullname: Yu, Jiyun |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23294724$$D View this record in MEDLINE/PubMed |
BookMark | eNo1j81qhDAUhbOY0plO5xVKXkCaxCRXl2WwPyAUil10NVzNFQWjYpR23r72Z1Zn8XEO37lhm37oacN2UgiIEoiTLTuE0JbCyBiMlPqabVWsUg1K79hHFmYsuzY0nvqZDzVHPi6dH3qcztzTjCsPbeB-cNT9cE8d9oNH_tnODf9tnzl9jROFQI43RfZW3OdLdcuuauwCHf5zz94fs-L4HOWvTy_HhzwapbJzZBF0nQJZAGdTY121iidOYmqdxdjViYpNJQwQSaGNcyBQGpRWA8rUodqzu7_dcSk9udM4tX51P10-qm-T1E_E |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
ExternalDocumentID | 23294724 |
Genre | English Abstract Journal Article |
GroupedDBID | --- -05 2B. 2C. 5XA 5XB 5XF 92F 92I ACGFS ALMA_UNASSIGNED_HOLDINGS CCEZO CGR CIEJG CUY CVF CW9 ECM EIF F5P NPM TCJ TGQ U1G U5K U5O |
ID | FETCH-LOGICAL-p126t-6a74f97e677d6956dc1008d1a96d6a3df8235c057ee1045dd70a15a1647a19da2 |
ISSN | 1007-8738 |
IngestDate | Tue Oct 15 23:45:26 EDT 2024 |
IsPeerReviewed | false |
IsScholarly | true |
Issue | 1 |
Language | Chinese |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-p126t-6a74f97e677d6956dc1008d1a96d6a3df8235c057ee1045dd70a15a1647a19da2 |
PMID | 23294724 |
ParticipantIDs | pubmed_primary_23294724 |
PublicationCentury | 2000 |
PublicationDate | 2013-Jan |
PublicationDateYYYYMMDD | 2013-01-01 |
PublicationDate_xml | – month: 01 year: 2013 text: 2013-Jan |
PublicationDecade | 2010 |
PublicationPlace | China |
PublicationPlace_xml | – name: China |
PublicationTitle | Xi bao yu fen zi mian yi xue za zhi |
PublicationTitleAlternate | Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi |
PublicationYear | 2013 |
SSID | ssib051375114 ssj0042138 ssib001104349 |
Score | 1.9682564 |
Snippet | To construct the eukaryotic expression vectors of human telomerase reverse transcriptase (hTERT) and luciferase (Luc) gene, and stably transfect B16 cell line... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 89 |
SubjectTerms | Animals Cell Line, Tumor Disease Models, Animal Gene Expression Humans Luciferases - genetics Lung Neoplasms - genetics Lung Neoplasms - secondary Male Melanoma, Experimental - genetics Melanoma, Experimental - pathology Mice Neoplasm Metastasis Plasmids - genetics Telomerase - genetics |
Title | Establishment of a pulmonary metastasis model of melanoma with stably expressed hTERT/Luc |
URI | https://www.ncbi.nlm.nih.gov/pubmed/23294724 |
Volume | 29 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3JTsMwELVYLlwQiH2TD9ysAtns5IgQi8pyKlJ7Qnbs0Eg0DVIj0Xw9YzsbCBBwiaIZxWn97MmbSWYGoWNgEIxKCr5JrCQ4KBFsqUAI3d9EJoGQUUh1gvP9A7159PvDYNi2OzLZJTNxEpdf5pX8B1WQAa46S_YPyDaDggDOAV84AsJw_BXGl0DtTBSpeaNP8uIFbq8_hZuoGQe9Ljhi2t3YN-kvPJtOePVlur56rov8mwrikoyB2w7g59wVcZe0DlMi-JTMC5KojJQpmWirME_JW6FIyUk5Tmvchik3sddRI7m2gj5c8pzXz8lWfls0q3NkY7H9NHtNu8EI3RiiCUZY-6kjnyGzBVtqA1uFNLoLyVpL2zyoA1Q-MUgBy4t8ZnOrf9Z-qpVdqxbRIgu11XvoxG6A6Phe62wGjseAbfr1c9t3Hc8mTlZ_QdeJrsb75HMY7jFYQ6uV04DP7QpYRwvleAONPqCPpwnmuEEft-hjg77W1-hjjT626OMGfWzQPwXsN9Hj1eXg4qZXNcro5Y5LZz3KmZ9ETFHGJAWHV8a6ZJN0eATbkHsyCV0viIGZKwVzEEjJzrgTcF1KjjuR5O4WWsqmmdpBmEWuBGHoAFX1Yy8RQRJRJrgnYEDu-bto287EU26roTzVc7T3rWYfrbRr5QAtJ7D91CFwuZk4MhC9A6vOSRQ |
link.rule.ids | 783 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Establishment+of+a+pulmonary+metastasis+model+of+melanoma+with+stably+expressed+hTERT%2FLuc&rft.jtitle=Xi+bao+yu+fen+zi+mian+yi+xue+za+zhi&rft.au=Xiao%2C+Yi&rft.au=Gao%2C+Jiangping&rft.au=Gao%2C+Kun&rft.au=Yan%2C+Jinqi&rft.date=2013-01-01&rft.issn=1007-8738&rft.volume=29&rft.issue=1&rft.spage=89&rft_id=info%3Apmid%2F23294724&rft_id=info%3Apmid%2F23294724&rft.externalDocID=23294724 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1007-8738&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1007-8738&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1007-8738&client=summon |