Development of a new dumbbell-shaped decoy DNA using a combination of the unnatural base pair ImO(N):NaN(O) and a CuAAC reaction

• Published in: Bioconjugate chemistry Vol. 25; no. 7; pp. 1360 - 1369
• Main Authors: Higuchi, Yosuke, Furukawa, Kazuhiro, Miyazawa, Tadashi, Minakawa, Noriaki
• Format: Journal Article
• Language: English
• Published: United States 16.07.2014
• Subjects: Alkynes - chemistry; Azides - chemistry; Base Pairing; Binding, Competitive; Copper - metabolism; Deoxyribonucleases - metabolism; DNA - chemistry; DNA - metabolism; Humans; NF-kappa B - antagonists & inhibitors; NF-kappa B - metabolism; Oligodeoxyribonucleotides - chemistry; Oligodeoxyribonucleotides - metabolism

We describe the synthesis and potential application of a new dumbbell-shaped decoy DNA prepared using a combination of the base pair ImO(N):NaN(O) and a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. The CuAAC reaction between the azido group on the 5'-end of oligodeoxynucleotide...