Value of multiparameter flow cytometry in the diagnosis and prognostic evaluation of childhood myelodysplastic syndrome

• Published in: Zhongguo dang dai er ke za zhi Vol. 20; no. 10; p. 819
• Main Authors: Liu, Chao, An, Wen-Bin, Zhang, Jing-Liao, Zhang, Ran-Ran, Sun, Cong-Cong, Chang, Li-Xian, Liu, Tian-Feng, Zou, Yao, Wang, Hui-Jun, Zhu, Xiao-Fan
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2018
• Subjects: Bone Marrow; Child; Flow Cytometry; Humans; Myelodysplastic Syndromes; Prognosis; Retrospective Studies
• ISSN: 1008-8830
• DOI: 10.7499/j.issn.1008-8830.2018.10.007

To investigate the value of multiparameter flow cytometry (MFC) and flow cytometric scoring system (FCSS) in the diagnosis and prognostic evaluation of childhood myelodysplastic syndrome (MDS). A retrospective analysis was performed for the clinical data of 42 children who were diagnosed with MDS. MFC was performed to investigate the phenotype and proportion of each lineage of bone marrow cells. The correlations of FCSS score with MDS type, International Prognostic Scoring System (IPSS) score, and revised IPSS (IPSS-R) score were analyzed. Of all the 42 children, 20 (48%) had an increase in abnormal marrow blasts, 19 (45%) had a lymphoid/myeloid ratio of >1, 14 (33%) had abnormal cross-lineage expression of lymphoid antigens in myeloid cells, 8 (19%) had abnormal CD13/CD16 differentiation antigens, 5 (12%) had abnormal expression of CD56, 3 (7%) had reduced or increased side scatter of granulocytes, 3 (7%) had reduced expression of CD36 in nucleated red blood cells, 2 (5%) had reduced expression of CD71 in nu