Influence of hairpin RNA, specific to the gene lawc, on expression of overlapping genes of the lawc/Trf2 complex in D. melanogaster

We used a genetic system of the gene complex leg-arista-wing complex/TBP related factor 2 (lawc/Trf2) as a suitable model for in vivo study of operational characteristics of divergent overlapping genes. We established lines of transgenic fruit flies carrying constructs capable of expressing hairpin...

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Published inIzvestiia Akademii nauk. Seriia biologicheskaia no. 2; p. 133
Main Authors Cherezov, R O, Vorontsova, Iu E, Mertsalov, I B, Kulikova, D A, Simonova, O B
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.2013
Subjects
Animals
Animals, Genetically Modified
Drosophila melanogaster - embryology
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Gene Expression Regulation, Developmental
Genes, Overlapping
RNA Interference
RNA, Small Interfering
Telomeric Repeat Binding Protein 2 - genetics
Telomeric Repeat Binding Protein 2 - metabolism
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Summary:We used a genetic system of the gene complex leg-arista-wing complex/TBP related factor 2 (lawc/Trf2) as a suitable model for in vivo study of operational characteristics of divergent overlapping genes. We established lines of transgenic fruit flies carrying constructs capable of expressing hairpin RNA directed at post-transcriptional suppression of the mRNA gene lawc by way of RNA-interference. The attempt to artificially suppress lawc-transcripts caused a rise in the level of expression of the gene lawc by several times. It is shown that change in the concentration of lawc-transcripts can affect the concentration of Trf2 transcripts. Possible mechanisms of regulation of expression of the overlapping genes studied are discussed.
ISSN:1026-3470