Coculture of stem cells from the apical papilla and human umbilical vein endothelial cells enhances the angiogenic potential of dental pulp tissues

• Published in: Shanghai kou qiang yi xue Vol. 23; no. 5; p. 547
• Main Authors: Yuan, Chang-yong, Wang, Peng-lai, Zhang, Cheng-fei, Liu, Zong-xiang, Luo, San-lian
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2014
• Subjects: Alkaline Phosphatase; Cell Differentiation; Cell Proliferation; Coculture Techniques; Dental Papilla; Dental Pulp; Human Umbilical Vein Endothelial Cells; Humans; Neovascularization, Physiologic; Odontogenesis; Organic Chemicals; Stem Cells
• ISSN: 1006-7248

To observe the effect of coculture of stem cells from the apical papilla (SCAPs) and human umbilical vein endothelial cells (HUVECs) on the angiogenic potential of dental pulp tissues. SCAPs were incubated in osteo/odontogenic, adipogenic, neurogenic induction medium and α-MEM medium, whose multilineage differentiation capacities were confirmed using alizarin red staining, oil red O staining and βIII-tubulin immunofluorescent staining. The tubular length, branching points number and junctional areas were detected after 3, 6, 9 h since cells were seeded onto matrigel, and the data was analyzed using SPSS 16.0 software package. SCAPs in the experimental groups were detected having more lipid droplets, mineralization nodules and neuron-like cells. Coculture of SCAPs and HUVECs formed more vessel-like structures in tubular formation assay. SCAPs are capable of differentiating into fat, bone, and nerve-like cells in vitro. Coculture of SCAPs and HUVECs can enhance the angiogenic potential of dental pulp tissues.