Establishment of a quality control system for HLA allele typing and its key points

• Published in: Zhonghua yi xue yi chuan xue za zhi Vol. 35; no. 3; p. 324
• Main Authors: Gao, Suqing, Xu, Yunping, Nie, Dongmei, Deng, Zhihui, Hong, Wenxu
• Format: Journal Article
• Language: Chinese
• Published: China 10.06.2018
• Subjects: Adult; Alleles; Base Sequence; DNA Primers - genetics; Exons; Female; Genotype; Histocompatibility Testing - methods; HLA-A Antigens - genetics; HLA-DRB1 Chains - genetics; Humans; Male; Molecular Sequence Data; Polymerase Chain Reaction; Young Adult
• DOI: 10.3760/cma.j.issn.1003-9406.2018.03.004
• ISSN: 1003-9406

To list the key points for quality control during HLA-A, B, C, DRB1 and DQB1 allele typing by taking consideration of hardware, software and experimental procedures. A total of 10 167 samples from randomly selected healthy blood donors and donor-recipient pairs from Shenzhen were typed for exons 2-4 of HLA-A, B, C, exon 2 of HLA-DRB1, and exons 2 and 3 of HLA-DQB1 by PCR- sequence-based typing. For 56 cases whose forward and reverse sequences were inconsistent, the samples were re-checked by a PCR-sequence specific oligonucleotide probe method. Novel alleles not included in the IMGT/HLA database were cloned and sequenced using in-house primers. Eight novel HLA alleles were identified. A table for key positions of single nucleotide polymorphisms (SNPs) were generated, which summarized the key points for quality control during HLA-A, B, C, DRB1 and DQB1 allele typing. Among the listed SNPs, 3 were located at the HLA-A locus, 8 were at the HLA-B locus, 6 were at the C locus, 6 were at the DQB1 locus, and 4 were