Establishment of a Xenografted Acute Myeloid Leukemia Model by using Zebrafish

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 25; no. 1; p. 35
• Main Authors: Ye, Yong-Bin, Zhang, Ming-Wan, Qiu, Da-Fa, Guo, Zi-Wen, He, Hui-Qing, Xu, Xiao-Jun
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2017
• Subjects: Animals; Cell Count; Disease Models, Animal; Heterografts; Humans; Leukemia, Myeloid, Acute; Zebrafish
• ISSN: 1009-2137
• DOI: 10.7534/j.issn.1009-2137.2017.01.006

Objectve: To investigate the feasibility of establishing xenografted leukemia model by zebrafish, so as to provide the more direct model in vitro and experimental evidence for study of acute myeloid leukemia and screening of the drugs for targeting therapy. Acute myeloid leukemia cell line KG-1a was labeled with red fluorescent dye-MitoRed, then the labeled cells were injected into the yolk sac of zebrafish embryos. Morphological observation, cell count and histopathological detection were used to analyse the infiltration and metastasis of KG-1a cells in zebrafish. KG1a cells could proliferate and gradually spread to the entire abdominal cavity of the zebrafish after KG-1a cells were injected into the yolk sac during 1-7, the results of cell counting in vitro also proved a significant proliferation of KG-1a cells in zebrafish, suggesting that the implanted leukemia stem cells could survive, proliferate and spread in zebrafish. Further study showed that the implanted cells could be transfered to the liver of z