Eficient inhibition of human influenza A virus by oligonucleotides electrostatically fixed on polylysine-containing TiO2 nanoparticles

• Published in: Bioorganicheskaia khimiia Vol. 40; no. 2; p. 196
• Main Authors: Levina, A S, Repkova, M N, Ismagilov, Z R, Shikina, N V, Mazurkova, N A, Zarytova, V F
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.03.2014
• Subjects: Antiviral Agents; Cell Line; Humans; Influenza A Virus, H3N2 Subtype - drug effects; Influenza A Virus, H3N2 Subtype - pathogenicity; Nanoparticles - administration & dosage; Nanoparticles - chemistry; Oligonucleotides - administration & dosage; Oligonucleotides - chemistry; Polylysine - chemistry; RNA, Viral - drug effects; Static Electricity; Titanium - administration & dosage; Titanium - chemistry
• ISSN: 0132-3423
• DOI: 10.7868/S0132342314020092

Antiviral activity of TiO2 * PL * DNA nanobiocomposites was studied on the MDCK cell culture infected with influenza A virus (subtype H3N2). DNA fragments in the nanocomposites are electrostatically bound to titanium dioxide nanoparticles pre-covered with polylysine. It was shown that TiO2 * PL * DNA(v3') nanocomposite bearing the DNA(v3') fragment targeted to the 3'-end of the noncoding region of segment 5 of viral RNA specifically inhibited the virus reproduction with the efficiency of 99.8 and 99.9% (or by factors of~400 and 1000) at a low concentration of DNA(v3') in nanocomposite (0.1 and 0.2 µM, respectively). The TiO2 * PL * DNA(r) nanocomposite containing oligonucleotide noncomplementary to viral RNA or the oligonucleotide unbound to the nanoparticles show very low antiviral activity (inhibition by factors of~3.5 and 1.3, respectively).