Rapid separation and determination of enterotoxigenic E. coli by capillary zone electrophoresis

Rapid separation and determination of enterotoxigenic E. coli K88, K99 and 987P with intact cells using capillary zone electrophoresis (CZE) are described. The CZE was performed in a running buffer of 0.05 mol/L Na2CO3-NaHCO3 (pH 9.9), under applied voltage of 14.1 kV and detected by an on-line UV a...

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Bibliographic Details
Published inSepu Vol. 20; no. 5; p. 439
Main Authors Chen, Ping, Li, Ren-Kuan, Xu, Xiao-Hua, Rao, Ping-Fan
Format Journal Article
LanguageChinese
Published China 01.09.2002
Subjects
Animals
Diarrhea - microbiology
Diarrhea - veterinary
Electrophoresis, Capillary - methods
Enterotoxigenic Escherichia coli - chemistry
Enterotoxigenic Escherichia coli - isolation & purification
Escherichia coli Infections - microbiology
Escherichia coli Infections - veterinary
Feces - chemistry
Feces - microbiology
Swine
Swine Diseases - microbiology
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Summary:Rapid separation and determination of enterotoxigenic E. coli K88, K99 and 987P with intact cells using capillary zone electrophoresis (CZE) are described. The CZE was performed in a running buffer of 0.05 mol/L Na2CO3-NaHCO3 (pH 9.9), under applied voltage of 14.1 kV and detected by an on-line UV at 210 nm. Results show that K88, K99 and 987P were separated completely. Moreover, each strain presented a characteristic peak and a reproducible retention time (RSD < or = 0.9%). The successful analysis of enterotoxigenic E. coli in the excrement of diarrhea piglets is also discussed. K88 and K99 were detected in the excrement of diarrhea piglets born in 5 d - 6 d, and 30 d - 35 d respectively, and nothing was detected in the excrement of diarrhea piglets born in about 60 d.
ISSN:1000-8713