Complete genome sequencing and analyses of rabies viruses isolated from wild animals (Chinese Ferret-Badger) in Zhejiang province

• Published in: Zhōnghuá liúxíngbìng zázhì Vol. 30; no. 8; p. 824
• Main Authors: Lei, Yong-Liang, Wang, Xiao-Guang, Liu, Fu-Ming, Chen, Xiu-Ying, Ye, Bi-Feng, Mei, Jian-Hua, Lan, Jin-Quan, Tang, Qing
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2009
• Subjects: Animals; Arvicolinae - virology; China; Deer - virology; Disease Reservoirs - veterinary; Disease Reservoirs - virology; Dogs - virology; Genome, Viral; Molecular Sequence Data; Mustelidae - virology; Mutation; Phylogeny; Rabies virus - genetics; Rabies virus - isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; Viral Proteins - genetics; China
• ISSN: 0254-6450

Based on sequencing the full-length genomes of two Chinese Ferret-Badger, we analyzed the properties of rabies viruses genetic variation in molecular level to get information on prevalence and variation of rabies viruses in Zhejiang, and to enrich the genome database of rabies viruses street strains isolated from Chinese wildlife. Overlapped fragments were amplified by RT-PCR and full-length genomes were assembled to analyze the nucleotide and deduced protein similarities and phylogenetic analyses of the N genes from Chinese Ferret-Badger, sika deer, vole, dog. Vaccine strains were then determined. The two full-length genomes were completely sequenced to find out that they had the same genetic structure with 11 923 nts including 58 nts-Leader, 1353 nts-NP, 894 nts-PP, 609 nts-MP, 1575 nts-GP, 6386 nts-LP, and 2, 5, 5 nts- intergenic regions (IGRs), 423 nts-Pseudogene-like sequence (Psi), 70 nts-Trailer. The two full-length genomes were in accordance with the properties of Rhabdoviridae Lyssa virus by blast an