Development and application of real-time polymerase chain reaction to detect Vibrio cholerae O1 and O139 in river water

• Published in: Zhōnghuá liúxíngbìng zázhì Vol. 28; no. 8; p. 768
• Main Authors: Wang, Xiao-Mei, Wang, Duo-Chun, Tan, Hai-Ling, Zhong, Hao-Jie, Chen, Jing-Diao, Li, Bai-Sheng, Ke, Chang-Wen, Yan, Mei-Ying, Zhang, Jing, Kan, Biao
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2007
• Subjects: Environmental Monitoring - methods; Genes, Bacterial; Polymerase Chain Reaction - methods; Reproducibility of Results; Rivers - microbiology; Sensitivity and Specificity; Vibrio cholerae O1 - isolation & purification; Vibrio cholerae O139 - isolation & purification

To develop a real-time SYBR Green polymerase chain reaction (PCR) for detection of Vibrio cholerae serogroups O1 and O139, and to evaluate its reliability through detection of estuary water samples. O antigen rfb genes specific for O1 and O139 were used for the design of PCR primers. The real-time S...