In vitro effect of Huyinling extract on Trichomonas tenax

The active ingredient of Huyinling, a combination of Chinese traditional medicine, was extracted by five different ethanol concentrations (40%-80%). There were seven groups named as five Huyinling ethanol extract groups (40%, 50%, 60%, 70%, and 80%), metronidazole group and blank control. Each Huyin...

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Published inZhongguo jishengchongxue yu jishengchongping zazhi Vol. 28; no. 5; p. 399
Main Authors Liang, Yu-Fen, Yun, Chen-Xia, Wei, Jun-Bin, Li, Qiang, Wang, Zhi-Ping
Format Journal Article
LanguageChinese
Published China 30.10.2010
Subjects
Antitrichomonal Agents - pharmacology
Drugs, Chinese Herbal - pharmacology
Trichomonas - drug effects
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Summary:The active ingredient of Huyinling, a combination of Chinese traditional medicine, was extracted by five different ethanol concentrations (40%-80%). There were seven groups named as five Huyinling ethanol extract groups (40%, 50%, 60%, 70%, and 80%), metronidazole group and blank control. Each Huyinling ethanol extract group was further divided into five subgroups with final concentration of 6.25, 12.5, 25, 50, and 100 mg/ml, respectively. Metronidazole group was given 10 microg/ml of the drug. Each group had 4 wells with 125 microl T. tenax(2 x 10(5)/ml). At 12 h, 24 h and 48 h after drug treatment, the anti-T. tenax effect of Huyinling ethanol extract was tested by microscope counting method. At 24 h the effect of Huyinling on T. tenax was examined with methyl thiazolyl tetrazolium (MTl) assay. The results showed that the higher concentration of Huyinling ethanol extract, the better effect on anti-T. tenax. 60% Huyinling ethanol extract group with concentrations of 6.25 mg/ml and 12.5 mg/ml showed higher an
ISSN:1000-7423