Effect of petroleum ether extract from Melilotus suaveolens Ledeb on the expression of NF-kappaB and Heme oxygenase 1

• Published in: Xi bao yu fen zi mian yi xue za zhi Vol. 24; no. 9; p. 861
• Main Authors: Pang, Ran, Zhang, Shu-ling, Zhao, Lei, Liu, Shuang-lin, Dong, Ji-hua, Tao, Jun-yan
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2008
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Cell Line, Tumor; Ether - chemistry; Gene Expression - drug effects; Heme Oxygenase-1 - genetics; Heme Oxygenase-1 - immunology; Inflammation - drug therapy; Inflammation - genetics; Inflammation - immunology; Melilotus - chemistry; Mice; NF-kappa B - genetics; NF-kappa B - immunology; Plant Extracts - pharmacology
• ISSN: 1007-8738

To study the anti-inflammatory effect of petroleum ether extract from Melilotus suaveolens Ledeb. Inflammatory cell model was constructed by LPS acting on the RAW264.7 cell line. The expression and distribution of NF-kappaB were detected using immunocytochemical method. The expression of mRNA and protein of Heme oxygenase 1(HO-1) were detected by real-time PCR and Western blot. The immunocytochemical analysis showed that the cytoplasm stained to brown presented NF-kappaB inactivation after the intervention of petroleum ether extract while the cell nucleus stained to brown presented NF-kappaB activation after the only intervention of LPS. The expression of HO-1 mRNA was significantly enhanced by the extract in a dose-dependent manner, and the expression of HO-1 protein was markedly enhanced too. The petroleum ether extract from Melilotus suaveolens Ledeb can resist inflammation by inhibiting the activation of proinflammatory factor NF-kappaB and enhancing the expression of anti-inflammatory factor HO-1.