Evaluation of immunization protection efficacy of plague subunit vaccine

• Published in: Zhōnghuá yùfáng-yīxué zázhì Vol. 43; no. 9; p. 785
• Main Authors: Zhang, Qing-wen, Qi, Zhi-zhen, Xin, You-quan, Yang, Yong-hai, Wu, Hai-lian, Yang, Han-qing, Feng, Jian-ping, Jin, Xing, Cui, Bai-zhong, Wang, Tang, Wu, Ben-chuan, Qiu, Ye-feng, Wang, Wang, Guo, Zhao-biao, Wang, Zu-yun, Yang, Rui-fu, Wang, Hu, Wang, Xiao-yi
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2009
• Subjects: Animals; Antibodies, Bacterial - blood; Dose-Response Relationship, Immunologic; Female; Guinea Pigs; Immunization; Immunoglobulin G - blood; Mice; Mice, Inbred BALB C; Models, Animal; Plague - prevention & control; Plague Vaccine - immunology; Rabbits; Vaccines, Subunit - immunology
• ISSN: 0253-9624

To evaluate the protective efficacy of plague subunit vaccine, BALB/c mice, guinea pigs and rabbits were used in this study. Groups of mice (10 per group), guinea pigs (14 per group) and rabbits (6 per group) were immunized with F1 + rV270 vaccine, EV76 vaccine and alum adjuvant by intramuscular route, respectively. Serum antibody titres of mice, guinea pigs and rabbits were determined by ELISA and the immunized animals were challenged with 10(6) CFU of Y. pestis strain 141 at the 8th week after the primary immunization. The immunized mice, guinea pigs or rabbits with subunit vaccine developed anti-F1 IgG titre of 41 587.3 +/- 2.1, 11 543.7 +/- 2.1 or 522.4 +/- 22.4 and elicited statistical anti-F1 IgG titre difference among them (F = 17.58, P < 0.01). The immunized mice, guinea pigs or rabbits with subunit vaccine had anti-rV270 IgG titre of 15 748.7 +/- 1.6, 12.6 +/- 1.4 or 1648.0 +/- 5.0 and induced statistical anti-rV270 IgG titre difference among them (F value was 16.34, P < 0.01). There was significant