Observation the electroporation of blood cells in vitro by scanning electron microscope

To observe changes on cell membrane in blood cells after they were been electrified. Blood were electrified for 5, 10, 20, 30 s, 1 min respectively, and Scanning electron microscope was used to detect the changes on their cell membranes. Pores were detected both on electrified erythrocytes and leuko...

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Bibliographic Details
Published inFa yi xue za zhi Vol. 22; no. 3; p. 177
Main Authors Wang, Ye, Liu, Min, Peng, Xue-Mei, Li, Fan, Zhang, Fu, Zhang, Kui, Liao, Zhi-Gang
Format Journal Article
LanguageChinese
Published China 01.06.2006
Subjects
Adult
Blood Cell Count
Cell Membrane - ultrastructure
Cell Membrane Permeability
Electroporation - methods
Erythrocytes - ultrastructure
Female
Humans
In Vitro Techniques
Leukocytes - ultrastructure
Male
Microscopy, Electron, Scanning
Middle Aged
Young Adult
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Summary:To observe changes on cell membrane in blood cells after they were been electrified. Blood were electrified for 5, 10, 20, 30 s, 1 min respectively, and Scanning electron microscope was used to detect the changes on their cell membranes. Pores were detected both on electrified erythrocytes and leukocytes with round or ellipse shapes. The erythrocytes often have one or more pores while the leukocytes often have more pores looked like cribble. The rates of perforated cells were increased with the prolonging time of electrification, 5 s with 6% and 1 min increased to 40%. Alternating current can cause the cell perforating, and the rates of perforated cell were increased with the prolonging time of electrification.
ISSN:1004-5619