Relationship between single nucleotide polymorphisms in the promoter of COX-2 gene and hereditariness to NAFLD

• Published in: Zhonghua gan zang bing za zhi Vol. 18; no. 10; p. 773
• Main Authors: Cao, Ming-bo, Yang, Yu-xiu, Dong, Lei
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2010
• Subjects: Adolescent; Adult; Aged; Alleles; Case-Control Studies; Cyclooxygenase 2 - genetics; Fatty Liver - genetics; Female; Genetic Predisposition to Disease; Genotype; Haplotypes; Humans; Male; Middle Aged; Non-alcoholic Fatty Liver Disease; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; Young Adult
• ISSN: 1007-3418
• DOI: 10.3760/cma.j.issn.1007-3418.2010.10.012

To study the relationship between COX-2 gene and hereditariness to Nonalcoholic fatty liver disease by detecting single nucleotide polymorphisms in the promoter of COX-2 gene. Genotypes of 200 case patients with NAFLD and 206 control subjects were examined by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). The DNA samples were extracted from the peripheral blood of all subjects. Two SNPs, -1195G more than A and -765 G more than C, were identified with frequencies of variant alleles 54% and 5% in patients with NAFLD and 48% and 2% in control, respectively. A case-control analysis revealed a 1.13-fold (95% CI = 1.01-2.46) and a 2.35-fold (95% CI = 1.17-3.65) excess risk of developing NAFLD for -1195AA or -765CG genotype carriers compared with noncarriers. Compared with G-1195-G-765 containing haplotype, a greater risk of developing NAFLD was observed for A-1195-G-765 (OR =1.42; 95% CI =1.11-1.63) and A-1195-C-765 (OR = 4.24; 95% CI =1.72-14.22) containing haplotypes. A great