Effects of injectio Salvia miltiorrhiza on gentamicin-induced free radical formation in guinea pig cochlea

To investigate the effects of injectio Salvia Miltiorrhiza (SM) on gentamicin (GM)-induced free radical formation in guinea pig cochlea, and to explore possible mechanisms on GM-induced ototoxicity. Biochemical assays of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in guinea...

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Published inZhongguo ying yong sheng li xue za zhi Vol. 20; no. 4; p. 406
Main Authors Wang, Ai-Mei, Tang, Hao, Shen, Jing, Cui, Cheng
Format Journal Article
LanguageChinese
Published China 01.11.2004
Subjects
Animals
Cochlea - drug effects
Cochlea - metabolism
Free Radicals - metabolism
Gentamicins - toxicity
Guinea Pigs
Lipid Peroxidation
Malondialdehyde - analysis
Salvia miltiorrhiza
Superoxide Dismutase - metabolism
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Summary:To investigate the effects of injectio Salvia Miltiorrhiza (SM) on gentamicin (GM)-induced free radical formation in guinea pig cochlea, and to explore possible mechanisms on GM-induced ototoxicity. Biochemical assays of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in guinea pig cochlea, combined with auditory brainstem response (ABR) measurement and transmission electron microscopic observation were used in this investigation. SOD activity was significantly declined while MDA content was distinctly increased in cochlear tissues after GM injection (P < 0.01). Moreover, they were well correlated with auditory function damage (|r| > 0.7). Co-treatment with SM evidently enhanced SOD activity and decreased MDA content (P < 0.01, P < 0.05). Furthermore, auditory function was markedly ameliorated. Morphological changes of cochlea were consistent with those of hearing function. Lipid peroxidation elicited by free radical was involved in GM-induced cochleotoxicity. SM might enhance SOD activi
ISSN:1000-6834