Identification of proteins overexpressed in malignant gastric tumors: comparison of the results of 2-De and bioinformatics search

• Published in: Molekuliarnaia biologiia Vol. 45; no. 4; p. 738
• Main Authors: Grigor'eva, E S, Bukurova, Iu A, Krasnov, G S, Afanas'ev, S G, Cherdyntseva, N V, Tuzikov, S A, Choĭnzonov, E L, Karpov, V L, Lisitsyn, N A, Beresten', S F
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.07.2011
• Subjects: Biomarkers, Tumor - analysis; Biomarkers, Tumor - biosynthesis; Biomarkers, Tumor - genetics; Cell Cycle Proteins - analysis; Cell Cycle Proteins - biosynthesis; Cell Cycle Proteins - genetics; Computational Biology; Electrophoresis, Gel, Two-Dimensional; Gene Expression Regulation, Neoplastic; Humans; Intestinal Neoplasms - genetics; Intestinal Neoplasms - metabolism; Neoplasm Proteins - analysis; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; Proteomics; S100 Calcium Binding Protein A6; S100 Proteins - analysis; S100 Proteins - biosynthesis; S100 Proteins - genetics; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; Superoxide Dismutase - analysis; Superoxide Dismutase - biosynthesis; Superoxide Dismutase - genetics; Thioredoxins - analysis; Thioredoxins - biosynthesis; Thioredoxins - genetics
• ISSN: 0026-8984

Comparison of protein expression in intestinal and diffuse stomach tumors by 2D gel electrophoresis led to identification of three proteins (SOD2, S100A6, and TXN), which are overexpressed in tumors as compared to normal controls. It was shown, that overexpression of proteins SOD2 and TXN occurs much more frequently in diffuse tumors than in intestinal ones. A control panel of eleven proteins overexpressed in stomach tumors has been selected based on the data of comparative 2D analysis described in the literature. Bioinformatics search for mRNAs encoding proteins from the control panel in Oncomine database (which contains the results of determination of mRNA transcription level in tumor vs. normal samples) demonstrated the coincidence of proteomic and transcriptomic data for seven out of 11 proteins.