Protective effect of the aerosol immunization of mice with the polycomponent vaccine Immunovac VP-4 after the challenge of the animals with Klebsiella pneumoniae virulent strain

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii no. 1; p. 20
• Main Authors: Solov'ev, K G, Kurbatova, E A, Egorova, N B, Uspenskaia, S N, Tret'iakova, A V, Varfolomeev, A N, Gruber, I M, Efremova, V N, Akhmatova, N K, Diadizhshev, N R, Akhmatova, I B, Diadishchev, N R, Semenova, I B, Cherkasova, L S, Semenov, B F, Borovik, R V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.01.2006
• Subjects: Aerosols; Animals; Antibodies, Bacterial - blood; Bacterial Vaccines - administration & dosage; Drug Evaluation, Preclinical; Female; Immunization Schedule; Klebsiella Infections - immunology; Klebsiella Infections - prevention & control; Klebsiella pneumoniae - immunology; Lymphocyte Activation; Lymphocytes - immunology; Male; Mice; Spleen - immunology; Vaccination; Vaccines, Combined - administration & dosage
• ISSN: 0372-9311

Used four schemes of the administration of the preparation with different time of the exposition of the animals in an aerosol chamber were tested with their subsequent intraperitoneal challenge with K. pneumoniae virulent strain K16. Irrespective of the number of immunization courses, the administration of the preparation made at intervals of 1 day, or daily, did not ensure any protective effect, but only led to an insignificant increase in their survival time in comparison with nonimmunized animals. After intervals between immunizations were increased to 3 days the protective effect of aerosol immumization was obtained (the survival rate was 65-80 % and considerably differed from that of the controls). The protective effect of aerosol immunization thus obtained was comparable with the effectiveness immunization made in a single subcutaneous injection. Aerosol immunization resulted in low antibody titers to the antigens contained in the vaccine, while after a single subcutaneous injection high antibody titers to Klebsiella and Proteus antigens were detected. The antigen-stimulated blast transformation of spleen lymphocytes in mice subjected to aerosol immunizations in 5 exposures was high. After subcutaneous immunization significant changes in such characteristics were detected on day 15. The data thus obtained were indicative of good prospects in the development Immunovac VP-4 as the medicinal form intended for use in aerosols.