Relationship of plasma homocysteine and folic acid levels and 5,10-methylenetetrahydrofolate reductase gene mutation with venous thromboembolism

• Published in: Chung-hua nei kʿo tsa chih Vol. 43; no. 8; p. 591
• Main Authors: Wang, Mei-tang, Li, Qiang, Han, Feng-lai, Yao, Xiao-peng
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2004
• Subjects: 5,10-Methylenetetrahydrofolate Reductase (FADH2) - genetics; Adult; Aged; Case-Control Studies; Female; Folic Acid - blood; Genotype; Homocysteine - blood; Humans; Male; Middle Aged; Mutation; Venous Thrombosis - blood; Venous Thrombosis - epidemiology; Venous Thrombosis - genetics
• ISSN: 0578-1426

To investigate the association between concentrations of plasma homocysteine and folic acid, 5,10-methylenetetrahydrofolate reductase (MTHFR) C667T mutation and venous thromboembolism (VTE) and to analyze the effect of MTHFR C667T mutation on concentrations of plasma homocysteine and folic acid. In 58 patients with VTE and 58 sex and age matched controls, epidemiological risk factors were surveyed. The concentration of plasma homocysteine was measured by high performance liquid chromatography, and the concentration of plasma folic acid was measured by radioimmunoassay. MTHFR C667T genotype was measured by PCR-RFLP. The concentrations of plasma homocysteine and folic acid showed significant difference between the case group and the control group (OR = 1.5, 95% CI: 1.216 - 2.213 and OR = 0.396, 95% CI: 0.149 - 0.709, respectively). There was no significant difference in the frequency of mutant alleles in site 667 of MTHFR gene between the cases and the controls (P > 0.05). The concentration of plasma folic acid