No association between 3 polymorphisms of transforming growth factor beta3 gene and essential hypertension in Chinese

To investigate possible association between the single nucleotide polymorphisms (SNPs) of transforming growth factor beta3 (TGF-beta3) gene and essential hypertension (EH) in Chinese. The promoter region, exons, as well as part of the introns of TGF-beta3 gene were sequenced by a fluorescent labelin...

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Published inZhōnghuá xīnxuèguănbìng zázhì Vol. 33; no. 2; p. 127
Main Authors Hu, Bang-Chuan, Chu, Shao-Li, Wang, Ji-Guang, Wang, Gu-Liang, Gao, Ping-Jin, Zhu, Ding-Liang
Format Journal Article
LanguageChinese
Published China 01.02.2005
Subjects
Aged
Asian Continental Ancestry Group - genetics
Case-Control Studies
Exons
Female
Genotype
Humans
Hypertension - genetics
Middle Aged
Polymorphism, Single Nucleotide
Transforming Growth Factor beta3 - genetics
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Summary:To investigate possible association between the single nucleotide polymorphisms (SNPs) of transforming growth factor beta3 (TGF-beta3) gene and essential hypertension (EH) in Chinese. The promoter region, exons, as well as part of the introns of TGF-beta3 gene were sequenced by a fluorescent labeling automatic sequencing method to detect and characterize the SNPs in 24 DNA samples from a Chinese population. Then we conducted a case-control study using 396 patients with hypertension (case) and 214 nomortensive subjects (control). The three SNPs including Thr63Asn, SS5608219 and SS5608220 were genotyped by PCR-RFLP or real-time allele-specific PCR in subjects studied. Seven SNPs in the exons, introns and 3'untranslated region (3'UTR) of TGF-beta3 gene were identified. Among them, 2 SNPs were found to be novel genetic variants and one of the two located in the exon 1 and produced substitution of amino acid. However, no differences were found between hypertensives and nomortensives in genotype distribution and al
ISSN:0253-3758