Confirmation of hydrazone formation in HYNIC-peptide conjugate preparation, and its hydrolysis during labeling with (99m)Tc

• Published in: Applied radiation and isotopes Vol. 65; no. 7; pp. 805 - 808
• Main Authors: Gandomkar, M, Najafi, R, Shafiei, M, Ebrahimi, S E S
• Format: Journal Article
• Language: English
• Published: England 01.07.2007
• Subjects: Chromatography, High Pressure Liquid; Hydrazines - chemistry; Hydrazones - chemistry; Nicotinic Acids - chemistry; Peptides - chemistry; Peptides - isolation & purification; Somatostatin - chemistry; Spectrometry, Mass, Electrospray Ionization; Technetium - chemistry
• ISSN: 0969-8043
• DOI: 10.1016/j.apradiso.2007.03.002

Because of its monodenticity, 6-hydrazinopyridine-3-carboxylic acid (HYNIC) is of interest as a bifunctional chelator for labeling peptide with (99m)Tc. Here, we confirm the formation of hydrazone in HYNIC-conjugated peptide. The preparative HPLC was used to purify the HYNIC conjugated somatostatin-based peptide and the result showed two peaks, even after two consecutive purifications. Analysis of these peaks by mass spectrometry indicated the presence of hydrazone, produced during preparation conjugate. Further, we have shown that presence of hydrazone really does not matter because under (99m)Tc-labeling conditions, hydrazone is hydrolyzed back to HYNIC that then chelates (99m)Tc. A HYNIC-peptide conjugate freeze-dried kit was also prepared in a mildly acidic or neutral condition with a final pH of 6-7. The kit was then labeled by (99m)Tc and incubated in 100 degrees C for 10min, and a labeling yield of >95% was obtained.