Comparative analysis of serologic typing and HLA-II typing by micro-PCR-SSP

• Published in: Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA Vol. 22; no. 3; p. 247
• Main Authors: Wu, Da-Lin, Ling, Han-Xin, Ding, Hong, Zhang, Yi
• Format: Journal Article
• Language: Chinese; English
• Published: China 01.03.2002
• Subjects: Alleles; Antibodies, Monoclonal - immunology; DNA - genetics; Gene Frequency; Genotype; Histocompatibility Antigens Class II - genetics; Histocompatibility Antigens Class II - immunology; Histocompatibility Testing - methods; HLA-DQ Antigens - genetics; HLA-DQ Antigens - immunology; HLA-DR Antigens - genetics; HLA-DR Antigens - immunology; Humans; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics; Polymerase Chain Reaction - methods

To evaluate the accuracy of polymerase chain reaction with sequence specific primers (PCR-SSP) in HLA-II genotyping and analyze the causes of the errors occurring during the genotyping. Blood samples were obtained form patients with chronic renal insufficiency, leukemia or thalassemia and also from...