Comparison of effects of MgCl2 and Gpp(NH)p on antagonist and agonist radioligand binding to adenosine A1 receptors

• Published in: Acta pharmacologica Sinica Vol. 24; no. 8; pp. 729 - 740
• Main Authors: Finlayson, Keith, Maemoto, Takuya, Butcher, Steven P, Sharkey, John, Olverman, Henry J
• Format: Journal Article
• Language: English
• Published: United States 01.08.2003
• Subjects: Adenosine - analogs & derivatives; Adenosine - pharmacology; Adenosine A1 Receptor Agonists; Adenosine A1 Receptor Antagonists; Animals; Cell Membrane - drug effects; Cell Membrane - metabolism; Cerebral Cortex - cytology; Guanylyl Imidodiphosphate - pharmacology; Magnesium Chloride - pharmacology; Male; Radioligand Assay; Rats; Rats, Sprague-Dawley; Receptor, Adenosine A1 - metabolism; Xanthines - pharmacology

To investigate modulation of antagonist and agonist binding to adenosine A1 receptors by MgCl2 and 5 -guanylimidodiphosphate (Gpp(NH)p) using rat brain membranes and the A1 antagonist [3H]-8-cyclopentyl-1,3-dipropylxanthine ([3H]DPCPX) and the A1 agonist [3H]-2-chloro-N6-cyclopentyladenosine ([3H]CC...