Expression and rapid one-step purification of biologically active His-tagged factor C by Ni(2+) affinity column chromatography
Factor C is an unusual extracellular protein capable of inducing cytodifferentiation in certain Streptomyces strains. The protein is produced by Streptomyces griseus 45H at such a low amount that the study of its mode of action was hindered by the shortage of purified protein. We report here the exp...
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Published in | FEMS microbiology letters Vol. 196; no. 2; pp. 223 - 227 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
15.03.2001
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Subjects | |
Online Access | Get full text |
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