Thiol-reactive agents biphasically regulate inositol 1,4,5-trisphosphate binding and Ca(2+) release activities in bovine adrenal cortex microsomes

• Published in: Endocrinology (Philadelphia) Vol. 142; no. 6; pp. 2614 - 2621
• Main Authors: Poirier, S N, Poitras, M, Laflamme, K, Guillemette, G
• Format: Journal Article
• Language: English
• Published: United States 01.06.2001
• Subjects: Adrenal Cortex - ultrastructure; Animals; Calcium - metabolism; Calcium Channels - drug effects; Calcium Channels - metabolism; Cattle; Dithiothreitol - pharmacology; Ethylmaleimide - pharmacology; Inositol 1,4,5-Trisphosphate - metabolism; Inositol 1,4,5-Trisphosphate Receptors; Kinetics; Microsomes - drug effects; Microsomes - metabolism; Receptors, Cytoplasmic and Nuclear - drug effects; Receptors, Cytoplasmic and Nuclear - metabolism; Sulfhydryl Reagents - pharmacology; Thimerosal - pharmacology

Within all endocrine cells, the inositol 1,4,5-trisphosphate (InsP(3)) receptor plays an important role in regulation of the intracellular Ca(2+) concentration. In the present study we showed that a single short-term treatment with either N-ethylmaleimide (known to decrease InsP(3) receptor activity...