Detection of Salmonella in food samples by the combination of immunomagnetic separation and PCR assay

• Published in: International microbiology Vol. 3; no. 4; p. 225
• Main Authors: Jeníková, G, Pazlarová, J, Demnerová, K
• Format: Journal Article
• Language: English
• Published: Switzerland 01.12.2000
• Subjects: Animals; Cheese - microbiology; Colony Count, Microbial; Eggs - microbiology; Filtration - methods; Food Microbiology; Immunomagnetic Separation - methods; Meat - microbiology; Polymerase Chain Reaction - methods; Salmonella enteritidis - genetics; Salmonella enteritidis - isolation & purification

A combination of immunomagnetic separation and polymerase chain reaction (IMS-PCR) was used to detect Salmonella in food samples. Pre-enrichment of samples was combined with filtration through a membrane for the removal of food debris. The IMS-PCR assay combines selective extraction of bacteria by s...