Development of gas chromatographic method for chiral analysis of acrylic ketone alcohol and acrylic ketone acetate

• Published in: Sepu Vol. 20; no. 4; p. 359
• Main Authors: Hu, Wei, Tang, Shi-ping, Yang, Li-rong, Huang, Rong-bin, Bi, Chun-hui
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2002
• Subjects: Acrylates - analysis; Acrylates - isolation & purification; Chromatography, Gas - methods; Insecticides - analysis; Insecticides - isolation & purification; Stereoisomerism
• ISSN: 1000-8713

A gas chromatographic method was developed to directly determine the enantiomers of acrylic ketone alcohol and acrylic ketone acetate. Two pairs of enantiomers were nearly baseline-separated by using Model HP-5890 Gas Chromatograph with flame ionization detector and WCOT CD-Chiral-DEX CB (30 m x 0.25 mm i.d.) column. The optimum conditions were the split ratio of 30:1 for the sample injected, the flow rates of 3.7 mL/min for the carrier gas (nitrogen), 60 mL/min for the hydrogen, and 250 mL/min for the oxygen, and temperatures of 148 degrees C for the column, 230 degrees C for the injector, and 230 degrees C for the detector. The linearity and reproducibility were satisfactory. The regression coefficients were over 0.9965. The RSDs obtained were less than 1.25%. The method was further used to trace the reactants and products, and to evaluate the enzyme catalyst activity and selectivity under different conditions. The results show that the method developed is very successful.