Determination of elastin-antielastin circulating immune complexes in serum of normal subjects and atherosclerotic patients

Healthy subjects of different ages and a group of atherosclerotic patients were tested for the presence of elastin-antielastin circulating immune complexes (CIC) in their sera. For the purpose the authors used a method based on sequential precipitation with rising concentrations of polyethylene glyc...

Full description

Saved in:
Bibliographic Details
Published inCor et vasa (English ed.) Vol. 33; no. 4; p. 282
Main Authors Baydanoff, S, Nicoloff, A G
Format Journal Article
LanguageEnglish
Published Czech Republic 1991
Subjects
Adolescent
Adult
Aged
Antigen-Antibody Complex - analysis
Arteriosclerosis - diagnosis
Arteriosclerosis - immunology
Child
Child, Preschool
Diagnosis, Differential
Elastin - immunology
Enzyme-Linked Immunosorbent Assay
Female
Humans
Infant
Male
Middle Aged
Reference Values
Online AccessGet more information

Cover

More Information
Summary:Healthy subjects of different ages and a group of atherosclerotic patients were tested for the presence of elastin-antielastin circulating immune complexes (CIC) in their sera. For the purpose the authors used a method based on sequential precipitation with rising concentrations of polyethylene glycol (PEG), dissociation of the precipitated CIC and further analysis of the resuspended precipitates for the presence of elastin derived peptides by an enzyme-linked immunosorbent assay (ELISA). Elastin-antielastin CIC were detected only in the serum of atherosclerotic patients and that of healthy subjects over 60. The elastin-antielastin CIC obtained from the serum of atherosclerotic patients were precipitated by higher PEG concentrations mainly and had a higher elastin content in comparison with those isolated from the serum of healthy persons over 60.
ISSN:0010-8650