Hemopoietic stromal precursors in long-term culture of bone marrow: II. Significance of initial packing for creating a hemopoietic microenvironment and maintaining stromal precursors in the culture

Conversion of bone marrow cells to a single cell suspension prevents them from creating a hemopoiesis maintaining adherent cell layer (ACL) in culture. The ACL of such cultures is devoid of hemopoietic stromal precursors capable of transferring the hemopoietic microenvironment on implantation under...

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Bibliographic Details
Published inExperimental hematology Vol. 11; no. 3; p. 243
Main Authors Chertkov, J L, Drize, N J, Gurevitch, O A
Format Journal Article
LanguageEnglish
Published Netherlands 01.03.1983
Subjects
Animals
Bone Marrow Cells
Cell Communication
Cell Differentiation
Cells, Cultured
Female
Fibroblasts - cytology
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - radiation effects
Mice
Mice, Inbred C57BL
Time Factors
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Summary:Conversion of bone marrow cells to a single cell suspension prevents them from creating a hemopoiesis maintaining adherent cell layer (ACL) in culture. The ACL of such cultures is devoid of hemopoietic stromal precursors capable of transferring the hemopoietic microenvironment on implantation under the renal capsule of syngeneic mice. The regeneration of ACL after injury is possible in 1-wk-old, but not older, Dexter-type bone marrow cultures. The results prove the significance of the initial packing of the bone marrow cells in the differentiation of the hemopoietic stromal precursors. These data contradict previous reports which concluded that the structural orderliness of stromal tissue need not be preserved for the hemopoietic microenvironment to be transferred.
ISSN:0301-472X
1873-2399