2',3'-dideoxynucleoside triphosphates (ddNTP) and di-2',3'-dideoxynucleoside tetraphosphates (ddNp4ddN) behave differently to the corresponding NTP and Np4N counterparts as substrates of firefly luciferase, dinucleoside tetraphosphatase and phosphodiesterases

• Published in: Biochimica et biophysica acta Vol. 1334; no. 2-3; p. 191
• Main Authors: Sillero, M A, Madrid, O, Zaera, E, Sillero, A
• Format: Journal Article
• Language: English
• Published: Netherlands 15.03.1997
• Subjects: Acid Anhydride Hydrolases - metabolism; Animals; Antiviral Agents - chemical synthesis; Cell Membrane - metabolism; Deoxyribonucleotides - metabolism; Dideoxynucleosides - metabolism; Dinucleoside Phosphates - biosynthesis; Dinucleoside Phosphates - metabolism; Liver - metabolism; Luciferases; Phosphoric Diester Hydrolases - metabolism; Rats

2',3'-Dideoxynucleosides (ddN) and their derivatives are currently used as antiretroviral compounds. Their active agents are the corresponding 2',3'-dideoxynucleoside triphosphates (ddNTPs) generated inside the cell by host kinases. Dinucleoside tetraphosphates (Np4Ns) are molecu...