Molecular Characterization of a Novel beta 1,3-Galactosyltransferase for Capsular Polysaccharide Synthesis by Streptococcus agalactiae Type Ib
A group B streptococcus, Streptococcus agalactiae type Ib, produces a high-molecular-weight polysaccharide consisting of the following pentasaccharide repeating unit: -> 4)-[ alpha -D-NeupNAc-(2 -> 3)- beta -D-Galp-(1 -> 3)- beta -D-GlcpNAc-(1 -> 3)]- beta -D-Galp-(1 -> 4)- beta -D-Gl...
Saved in:
Published in | Journal of biochemistry (Tokyo) Vol. 131; no. 2; pp. 183 - 191 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.02.2002
|
Online Access | Get full text |
Cover
Loading…
Abstract | A group B streptococcus, Streptococcus agalactiae type Ib, produces a high-molecular-weight polysaccharide consisting of the following pentasaccharide repeating unit: -> 4)-[ alpha -D-NeupNAc-(2 -> 3)- beta -D-Galp-(1 -> 3)- beta -D-GlcpNAc-(1 -> 3)]- beta -D-Galp-(1 -> 4)- beta -D-Glcp-(1 ->. The type-specific capsular polysaccharide (CP) synthesis (cps) genes of this strain were cloned and analyzed. A cloned 10-kb DNA fragment contained cpslbE to L and neu (neuraminic acid synthesis gene) B. Comparison of the gene products with those of S. agalactiae type Ia, which has a similar but distinct CP, showed that the translation products of cpsla and cpslb genes exhibited very high homology except for those of cpsJ and K. In the type Ia strain, cpslaJ encodes beta 1,4-galactosyltransferase, which catalyzes the transfer of galactose as the fourth monosaccharide of the sugar repeating unit. In the type Ib CP, this galactose forms a beta 1,3-linkage to GlcNAc. The low homology between the type Ia and Ib CpsJs seems to reflect this difference. By enzymatic activity measurement, the cpslbJ product was found to display beta 1,3-galactosyltransferase activity. Furthermore, hydrophobic cluster analysis clarified the similarities and differences of the structures in N-terminal regions, including the DXD motif, between the galactosyltransferases. |
---|---|
AbstractList | A group B streptococcus, Streptococcus agalactiae type Ib, produces a high-molecular-weight polysaccharide consisting of the following pentasaccharide repeating unit: -> 4)-[ alpha -D-NeupNAc-(2 -> 3)- beta -D-Galp-(1 -> 3)- beta -D-GlcpNAc-(1 -> 3)]- beta -D-Galp-(1 -> 4)- beta -D-Glcp-(1 ->. The type-specific capsular polysaccharide (CP) synthesis (cps) genes of this strain were cloned and analyzed. A cloned 10-kb DNA fragment contained cpslbE to L and neu (neuraminic acid synthesis gene) B. Comparison of the gene products with those of S. agalactiae type Ia, which has a similar but distinct CP, showed that the translation products of cpsla and cpslb genes exhibited very high homology except for those of cpsJ and K. In the type Ia strain, cpslaJ encodes beta 1,4-galactosyltransferase, which catalyzes the transfer of galactose as the fourth monosaccharide of the sugar repeating unit. In the type Ib CP, this galactose forms a beta 1,3-linkage to GlcNAc. The low homology between the type Ia and Ib CpsJs seems to reflect this difference. By enzymatic activity measurement, the cpslbJ product was found to display beta 1,3-galactosyltransferase activity. Furthermore, hydrophobic cluster analysis clarified the similarities and differences of the structures in N-terminal regions, including the DXD motif, between the galactosyltransferases. |
Author | Watanabe, Masaki Miyake, Katsuhide Kataoka, Yohei Iijima, Shinji Endo, Tetsuo Koizumi, Satoshi Ozaki, Akio Yanae, Kouji |
Author_xml | – sequence: 1 givenname: Masaki surname: Watanabe fullname: Watanabe, Masaki – sequence: 2 givenname: Katsuhide surname: Miyake fullname: Miyake, Katsuhide – sequence: 3 givenname: Kouji surname: Yanae fullname: Yanae, Kouji – sequence: 4 givenname: Yohei surname: Kataoka fullname: Kataoka, Yohei – sequence: 5 givenname: Satoshi surname: Koizumi fullname: Koizumi, Satoshi – sequence: 6 givenname: Tetsuo surname: Endo fullname: Endo, Tetsuo – sequence: 7 givenname: Akio surname: Ozaki fullname: Ozaki, Akio – sequence: 8 givenname: Shinji surname: Iijima fullname: Iijima, Shinji |
BookMark | eNotjstOwzAURL0oEm3hH7yBFSlx3LyWKIJSqTykFolddX1zQ1O5cbAdRPgIvplQWI1GOjozEzZqTEOMXYhwJsJcXpvPythybzrbgHazvcIdHWYQhjLMkhEbh2Ekgjyav56yiXP73xpJOWbfD0YTdhosL3ZgAT3Z-gt8bRpuKg780XyQ5oo8cHElgwXogTGu195C4yqy4IgP27yA1h09z0b3DhAHXV0SX_eN35GrHVc9X3tLrTdoEDvH4e1oq4H4pm-JL9UZO6mG_3T-n1P2cne7Ke6D1dNiWdysglaIxAe5ElkZU5qghBQjiIXMS5khwjyOS6xSpaCKZBlJzEQyADllKiGRo0qpRCGn7PLP21rz3pHz20PtkLSGhkzntiKbiziJI_kDZuNyFQ |
ContentType | Journal Article |
DBID | 7QL C1K |
DOI | 10.1093/oxfordjournals.jbchem.a003086 |
DatabaseName | Bacteriology Abstracts (Microbiology B) Environmental Sciences and Pollution Management |
DatabaseTitle | Bacteriology Abstracts (Microbiology B) Environmental Sciences and Pollution Management |
DatabaseTitleList | Bacteriology Abstracts (Microbiology B) |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Anatomy & Physiology |
EndPage | 191 |
GroupedDBID | --- -E4 -~X .2P .55 .CO .GJ .I3 0R~ 18M 1TH 2WC 3O- 482 48X 53G 5GY 5RE 5WA 5WD 70D 7QL A8Z AAIMJ AAJKP AAMDB AAMVS AAOGV AAPQZ AAPXW AARHZ AASNB AAUAY AAUQX AAVAP AAVLN AAYJJ ABDBF ABEFU ABEUO ABIXL ABJNI ABMNT ABNKS ABPTD ABQLI ABQTQ ABSAR ABWST ABXVV ABZBJ ACGFO ACGFS ACIWK ACNCT ACPRK ACUFI ADBBV ADEYI ADEZT ADFTL ADGKP ADGZP ADHKW ADHZD ADIPN ADOCK ADQBN ADRIX ADRTK ADVEK ADYVW ADZTZ ADZXQ AEGPL AEGXH AEJOX AEKSI AELWJ AEMDU AENEX AENZO AEPUE AETBJ AEWNT AFFNX AFFZL AFGWE AFIYH AFOFC AFRAH AFXEN AGINJ AGKEF AGQXC AGSYK AHXPO AI. AIAGR AIJHB AJEEA AKHUL AKWXX ALMA_UNASSIGNED_HOLDINGS ALUQC APIBT APWMN ARIXL ASPBG ATGXG AVWKF AXUDD AYOIW AZFZN BAYMD BCRHZ BEYMZ BHONS BQDIO BSWAC C1A C1K CDBKE CS3 CZ4 DAKXR DILTD D~K EBD EBS EE~ EJD EMOBN ESTFP ESX F5P F9B FEDTE FHSFR FLUFQ FOEOM FQBLK GAUVT GJXCC H5~ HAR HH5 HVGLF HW0 HZ~ H~9 IOX J21 JSF JSH KAQDR KBUDW KOP KQ8 KSI KSN L7B M-Z N9A NGC NLBLG NOMLY NVLIB O0~ O9- OAWHX OBOKY ODMLO OJQWA OJZSN OK1 OWPYF P2P PAFKI PB- PEELM PQQKQ Q1. Q5Y R44 RD5 RJT RNI ROL ROX ROZ RUSNO RW1 RXO RZF RZJ RZO SJN SV3 TLC TN5 TUS TWZ UQL VH1 WH7 WHG X7H X7M YAYTL YKOAZ YROCO YXANX ZE2 ZGI ZKX ~91 ~KM |
ID | FETCH-LOGICAL-p116t-9b18d5e76c3a7c2a5139d38cca455dcf7bbaf23d23c816c2a9e8b6e19cb7edc13 |
ISSN | 0021-924X |
IngestDate | Fri Aug 16 21:44:52 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 2 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-p116t-9b18d5e76c3a7c2a5139d38cca455dcf7bbaf23d23c816c2a9e8b6e19cb7edc13 |
Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
PQID | 18415652 |
PQPubID | 23462 |
PageCount | 9 |
ParticipantIDs | proquest_miscellaneous_18415652 |
PublicationCentury | 2000 |
PublicationDate | 20020201 |
PublicationDateYYYYMMDD | 2002-02-01 |
PublicationDate_xml | – month: 02 year: 2002 text: 20020201 day: 01 |
PublicationDecade | 2000 |
PublicationTitle | Journal of biochemistry (Tokyo) |
PublicationYear | 2002 |
SSID | ssj0021233 |
Score | 1.8010689 |
Snippet | A group B streptococcus, Streptococcus agalactiae type Ib, produces a high-molecular-weight polysaccharide consisting of the following pentasaccharide... |
SourceID | proquest |
SourceType | Aggregation Database |
StartPage | 183 |
Title | Molecular Characterization of a Novel beta 1,3-Galactosyltransferase for Capsular Polysaccharide Synthesis by Streptococcus agalactiae Type Ib |
URI | https://search.proquest.com/docview/18415652 |
Volume | 131 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwFLbKkBAvCBiIO34AXkrKHOfWx6na6GArSLSie6qOY4dlK_G0JEjZj-Bf8D85dtK0ZRu3lyhKHCfK9-mcz_bxOYS8QBcmFVPSka6XOB7bip0IZOgkPEA6BVxwW5LlYBQMJ967qT_tdH6sRC2VhejF55fuK_kfVPEa4mp2yf4Dsm2neAHPEV88IsJ4_CuMDxa1be2qeZ13-bzVgNAd6W9qbkKxoIsGa8CdtzA39XXyal5YwarO0InZSMMB4HDZ9PRRz6scYrMbK5Wq-6nKUCKarCWoU80S9mmh0YbGZd6FL7a3FFTXjGa7e-IKpStSU5arritnFO1Yn1R6ZQriM6BCBVHPkUOOmralQVpBHT30Hoq8PEply8NDfKS-o8vj9gFsBvrECuJDfaTStUkNdxEHvTTUJnLErYM3W0PN2Qoj3RWzy-piOI0HZ3X9rwvOoU6cpe1GzAbFvHcszB_ogU3cc0lS7tGH2e5kf3823pmOr5Hrbtj3bTLh4TKlIzr_OpCh-eYb5GXzuje_fdkF9281zfg2udVARLdrZt0hHZXdJZvbGRT6a0VfURsebNddNsn3lmz0V7JRnVCglmzUkI2y11dQjeJ30gXV6DrVaEs1Kiq6RjW6pBo1VKN74h6Z7O6MB0OnKebhnDIWFE5fsEj6KgxiDmHsgo9DD8kjNCAemos4CYWAxOXS5XHEAmzQV5EIFOvHIlQyZvw-2ch0ph4QmgQB-EJyvAWegqQvPBZz5QrU8pFU8UPyfPFfZ8htswIGmdJlPmORma_w3Ud_bPGY3Fzy8gnZKM5K9RTlZyGeWfB_AlOGlLk |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | ABC ChemistRy |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Molecular+Characterization+of+a+Novel+beta+1%2C3-Galactosyltransferase+for+Capsular+Polysaccharide+Synthesis+by+Streptococcus+agalactiae+Type+Ib&rft.jtitle=Journal+of+biochemistry+%28Tokyo%29&rft.au=Watanabe%2C+Masaki&rft.au=Miyake%2C+Katsuhide&rft.au=Yanae%2C+Kouji&rft.au=Kataoka%2C+Yohei&rft.date=2002-02-01&rft.issn=0021-924X&rft.volume=131&rft.issue=2&rft.spage=183&rft.epage=191&rft_id=info:doi/10.1093%2Foxfordjournals.jbchem.a003086&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0021-924X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0021-924X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0021-924X&client=summon |