Modulation of Antigenic Phenotype in Cultured Human Osteoblast-like Cells by FGFb, TGF beta 1, PDGF-BB, IL-2, IL-1 beta , LPS and IFN gamma

• Published in: Bioscience reports Vol. 26; no. 4; pp. 281 - 289
• Main Authors: Perez, E, Garcia-Martinez, O, Arroyo-Morales, M, Reyes-Botella, C, Ruiz, C
• Format: Journal Article
• Language: English
• Published: 01.08.2006
• ISSN: 0144-8463
• DOI: 10.1007/s10540-006-9022-z

Background/Aims: Recent reports demonstrated that osteoblast-like cells can also exert activities directly associated with the immune system (cytokine synthesis, antigen presentation, phagocytosis and stimulation of T lymphocytes). The present study aimed to analyze the effect of Transforming growth factor beta 1 (TGF beta 1), Fibroblast growth factor basic (FGFb), Platelet-derived growth factor-BB (PDGF-BB), Interleukin-1 beta (IL-1 beta ), Interleukin-2 (IL-2), Lipopolysaccharide (LPS) and Interferon- gamma (IFN gamma ) on the expression on osteoblast-like cells of antigens involved in antigen presentation. Methods: Flow cytometry was used to investigate whether the growth factors FGFb, TGF beta 1, PDGF-BB, IL-2, IL-1 beta , LPS and IFN gamma modulate the expression on cultured human osteoblast-like cells of different antigens involved in antigen-presentation and T cell activation. Results: TGF beta 1 treatment significantly reduced the expression of CD54 and CD86. IL-1 beta treatment significantly enhanced the expression of CD54, CD86 and HLA-DR. LPS and IFN gamma treatments produced a major increase in CD54, CD80, CD86 and HLA-DR expression. Expression of these antigen-presenting molecules was not significantly modified by FGFb, PDGF-BB or IL-2 treatment.