Diagnostic performance of Leishmania braziliensis and Leishmania peruviana antigens in the immunoblot method for the detection of american tegumentary leishmaniasis

Motivation for the study. To contribute to the immunogenic character of soluble and excretion/secretion antigens of Leishmania braziliensis and Leishmania peruviana with the aim of identifying proteins with diagnostic potential. Main findings. The soluble antigen of Leishmania braziliensis has a sen...

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Published inRevista peruana de medicina experimental y salud pública Vol. 41; no. 3; pp. 294 - 300
Main Authors Rojas-Palomino, Nyshon, Sandoval-Juarez, Aidé, Solis-Sánchez, Gilmer, Minaya-Gómez, Gloria
Format Journal Article
LanguageEnglish
Spanish
Published Peru Instituto Nacional de Salud 21.10.2024
Subjects
Adult
Antigens, Protozoan - immunology
Brief Report
Female
Humans
Immunoblotting - methods
Leishmania braziliensis - immunology
Leishmaniasis, Cutaneous - diagnosis
Male
Middle Aged
Sensitivity and Specificity
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Summary:Motivation for the study. To contribute to the immunogenic character of soluble and excretion/secretion antigens of Leishmania braziliensis and Leishmania peruviana with the aim of identifying proteins with diagnostic potential. Main findings. The soluble antigen of Leishmania braziliensis has a sensitivity in the detection of ATL of 87.7%, specificity of 100% and a false positive rate of 20% against sera from patients with Chagas disease and 8.3% with mycosis. Implications. Immunoblot can improve the resolution capacity in the serological diagnosis of American tegumentary Leishmaniasis, particularly in patients where the length of the disease and the clinical form make difficult the diagnosis by parasitological methods. This study aimed to determine the performance of Leishmania braziliensis and Leishmania peruviana antigens in the detection of ATL by using serum samples obtained between 2013 - 2016. The obtained soluble and excretion/secretion antigens were transferred to membrane nitrocellulose by immunoblot assay. The evaluation was carried out against sera confirmed for ATL, at a confidence level of 95%, determining that the soluble antigen of Leishmania braziliensis had a sensitivity of 87.7%, specificity of 100% and area under the curve of 0.95; on the other hand, Leishmania peruviana showed values of 92.3%, 95.7% and 0.94, respectively. According to the results, we recommend that the reported immunogenic regions should be characterized and analyzed in order to continue with the development of recombinant and synthetic proteins, aimed at improving the efficiency of the serological diagnosis of the disease.
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Author contributions.: The authors declare that they each meet the four ICMJE criteria for authorship.
Roles according to CRediT.: NRP: conceptualization, methodology, formal analysis, research, resources, data curation, writing the original draft, reviewing and editing, project management. ASJ: research, resources, writing original draft, reviewing and editing, and fund acquisition. GSS: Data curation, formal analysis, writing the original draft, review and editing. GMG: Conceptualization, methodology, data curation, writing the original draft, review and editing, project management, and fund acquisition.
Conflict of interest.: NRP, ADJ and GMG are employees of the National Center for Public Health of the National Institute of Health (funding entity) and declare that they have no conflict of interest. GSS declares no conflict of interest.
ISSN:1726-4642
1726-4634
1726-4642
DOI:10.17843/rpmesp.2024.413.13231