Livin Regulates H2A.X Y142 Phosphorylation and Promotes Autophagy in Colon Cancer Cells via a Novel Kinase Activity
To investigate Livin-mediated regulation of H2A.X phosphorylation via a novel kinase activity and its effect on autophagy in colon cancer cells. The interaction between Livin and H2A.X was tested by immunoprecipitation. H2A.X-/- HCT116 cells were transfected with human influenza hemagglutinin (HA)-t...
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Published in | Frontiers in oncology Vol. 9; p. 1233 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
14.11.2019
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Subjects | |
Online Access | Get full text |
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Summary: | To investigate Livin-mediated regulation of H2A.X
phosphorylation via a novel kinase activity and its effect on autophagy in colon cancer cells.
The interaction between Livin and H2A.X was tested by immunoprecipitation. H2A.X-/- HCT116 cells were transfected with human influenza hemagglutinin (HA)-tagged WT or Y142F phospho-dead mutantH2A.X plasmids. GST-tagged recombinant Livin protein was used to perform
pull-down experiment and kinase assay. H2A.X-/-Livin+/+ SW480 cells were co-transfected with H2A.X
/H2A.X
plasmid and LC3 EGFP-tagged plasmid to explore whether H2A.X
was involved in Livin-mediated autophagy induced by starvation in colon cancer cells.
Co-immunoprecipitation studies confirmed that Livin interacted with H2A.X and that it was phosphorylation dependent.
kinase assay confirmed that Livin could phosphorylate H2A.X. Knockdown of Livin (Livin-/-) in SW480 cells or HCT116 cells canceled the starvation-induced autophagy in colon cancer cells; H2A.X-/-Livin+/+ SW480 cells transfected with H2A.X
activated autophagy induced by starvation while cells transfected with H2A.X
had no significant difference; Livin-H2A.X
axis activated autophagy in colon cancer cells through transcriptionally regulating
and
.
Livin promotes autophagy in colon cancer cells via regulating the phosphorylation of H2A.X
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ISSN: | 2234-943X 2234-943X |
DOI: | 10.3389/fonc.2019.01233 |