Purification of a novel G-protein alpha 0-subtype from mammalian brain

• Published in: Biochemical journal Vol. 300 ( Pt 2); no. Pt 2; pp. 387 - 394
• Main Authors: Nürnberg, B, Spicher, K, Harhammer, R, Bosserhoff, A, Frank, R, Hilz, H, Schultz, G
• Format: Journal Article
• Language: English
• Published: England 01.06.1994
• Subjects: Adenosine Diphosphate Ribose - metabolism; Amino Acid Sequence; Animals; Blotting, Western; Brain - drug effects; Brain Chemistry; Cattle; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; GTP-Binding Proteins - isolation & purification; Molecular Sequence Data; Pertussis Toxin; Precipitin Tests; Sequence Homology, Amino Acid; Swine; Virulence Factors, Bordetella - pharmacology

Three distinct G-protein alpha o-subtypes, i.e. alpha o1, alpha o2 and a newly observed 'alpha o3', are present in membranes of mammalian brain, each appearing as two isoforms on SDS/PAGE. Only alpha o1 and alpha o2 appear to be substrates for pertussis toxin (PTX) when membranes or partia...