Post-transcriptional modification of the wobble nucleotide in anticodon-substituted yeast tRNAArgII after microinjection into Xenopus laevis oocytes

• Published in: Nucleic acids research Vol. 11; no. 3; pp. 707 - 718
• Main Authors: Fournier, M, Haumont, E, de Henau, S, Gangloff, J, Grosjean, H
• Format: Journal Article
• Language: English
• Published: England 11.02.1983
• Subjects: Animals; Anticodon - genetics; Base Sequence; Endonucleases; Female; Kinetics; Microinjections; Nucleic Acid Conformation; Oocytes - metabolism; Ovum - metabolism; Ribonuclease T1; RNA Processing, Post-Transcriptional; RNA, Transfer - genetics; RNA, Transfer, Amino Acyl - genetics; Saccharomyces cerevisiae - genetics; Single-Strand Specific DNA and RNA Endonucleases; Xenopus

An enzymatic procedure for the replacement of the ICG anticodon of yeast tRNAArgII by NCG trinucleotide (N = A, C, G or U) is described. Partial digestion with S1-nuclease and T1-RNAase provides fragments which, when annealed together, form an "anticodon-deprived" yeast tRNAArgII. A novel...