A chemically synthesized radiolabeled signal peptide: design, preparation, and Biological evaluation of an iodinated analog of preproparathyroid hormone

• Published in: Molecular endocrinology (Baltimore, Md.) Vol. 2; no. 5; pp. 452 - 458
• Main Authors: CAULFIELD, M. P, DUONG, L. T, O'BRIEN, R, MAJZOUB, J. A, ROSENBLATT, M
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 01.05.1988
• Subjects: Amino Acid Sequence; Biological and medical sciences; Cross-Linking Reagents; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Humans; Iodine - pharmacology; Iodine Radioisotopes; Isotope Labeling; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Parathyroid Hormone - chemical synthesis; Parathyroid Hormone - pharmacology; Protein Precursors - chemical synthesis; Protein Precursors - pharmacology; Protein Sorting Signals - chemical synthesis; Protein Sorting Signals - pharmacology; Structure-Activity Relationship; Sulfur - pharmacology; Translation. Translation factors. Protein processing; Iodination; Leader peptide; Molecular interaction; Preproparathormone; Radiolabelling; Chemical synthesis

The chemically synthesized signal peptide (native-sequence signal peptide) of preproparathyroid hormone exhibits signal sequence-like activity by inhibiting the translocation/processing of precursor proteins to their mature forms in an in vitro translation system. In order to prepare a biologically...